Aurora Biomed has announced that its Versa LLE workstation has been used to perform 25-hydroxy vitamin D extraction.
Vitamin D deficiency is associated with many pathological conditions including diabetes, osteoarthritis and many forms of cancer.
Several methods are available to assess vitamin D sufficiency through measurement of serum 25-hydroxy vitamin D [25(OH)D].
Antibody (Elisa) or other protein-binding assays are available, but may not accurately report total vitamin D values.
Moreover, manual sample preparation methods for assays such as LC-MS/MS or HPLC are very cumbersome.
Aurora's Versa LLE workstation has been validated for the organic solvent extraction of 25(OH)D from blood serum using liquid-liquid extraction (partition chromatography).
Reliable reproducibility of peak height data from the extractions suggests that biomolecules such as 25(OH)D can be effectively extracted using this automation within acceptable values.
Moreover, the automated process takes 60 minutes for a set of 96 samples with no detectable cross contamination.
The extraction process took approximately 17 minutes less than the manual procedure.
