The SsoFast Evagreen Supermix with low ROX enhances the speed, reliability and sensitivity of qPCR experiments performed on instruments requiring ROX reference dye.
The ROX-blended version of the SsoFast Evagreen supermix is optimised for users of PCR systems that employ ROX as a passive reference for normalisation.
It can be used on Bio-Rad's real-time PCR detection systems, as well as on the ABI 7500 Fast and Standard, ABI Stepone and Steponeplus, and Stratagene MX-series real-time PCR systems.
'By pre-blending ROX into the SsoFast Evagreen supermix, ABI 7500 users can experience the same performance advantages achieved with our SsoFast Evagreen supermix,' said Viresh Patel, Bio-Rad senior product manager, PCR Reagents.
Through instant polymerase activation, optimal primer binding and rapid polymerisation kinetics, SsoFast Evagreen supermix with low ROX can help researchers reduce time to results from 90 minutes or more for standard cycling to between 35 and 45 minutes.
Under these fast qPCR conditions, real-time PCR experiments maintain broad dynamic range, reliability and accurate quantification.
The patented Sso7d-fusion polymerase utilises the dsDNA-binding protein Sso7d to enable fast cycling without affecting PCR sensitivity, efficiency or reproducibility.
In addition, the Sso7d-fusion polymerase is significantly more resistant to PCR inhibitors, making SsoFast Evagreen supermix an ideal choice for applications such as qPCR directly from unpurified sample (direct PCR).