Softgenetics has announced the latest release of its genotyping program, Genemarker, with the additions of modules for CF analysis, macromolecules and two new sizing algorithms.
The latest version of the software incorporates a module required for the comparative analysis of cystic fibrosis, wild type vs patient for use with Genprobe's Elucigene CF kit, as well as rapid visualisation of mutant alleles generated by the Abbott Cystic Fibrosis Genotyping assay.
Use of Genemarker for these and other clinical research assays such as MLPA, Trisomy, MS-MLPA eliminates the need for tedious and error-prone data transfer and manipulation, as Genemarker performs the required sizing, normalisation and analysis in a single integrated software package.
Genemarker includes a user management tool that controls access rights and provides an analysis audit trail.
The latest Genemarker version also features a tool for the analysis of macromolecules, such as carbohydrates, polysaccharides, proteins and polypeptides, without the use of an internal size standard.
The macromolecule analysis tool can be used as a low-cost method for frameshift assays, as well as other food science assays of CE data.
Two new sizing algorithms have been incorporated into the software to allow for linear sizing of DNA fragments from 20bp to more than 1,000bp, plus an algorithm for the sizing of non-linear data with agarose gels and very extreme size ranges.
Traditional sizing methods, Local Southern and Cubic Spline are problematic for obtaining accurate size calling of fragments more than 800bp.
The large-size algorithm affords many time and cost-reducing multiplexing opportunities such as the addition of four probes to clinical assays, forensic profiling and ecology analysis.
