The Macherey-Nagel Nucleospin miRNA kit for gene expression studies is now available to UK scientists through Thermo Fisher Scientific.
The RNA extraction kit has the flexibility to fractionate RNA by size and can provide a total protein fraction.
The company said this ensures highly reliable results in gene expression studies.
This allows gene expression to be analysed on the transcription level (mRNA and miRNA) as well as on the level of translated protein.
Nucleospin miRNA can be used to isolate excellent yields of small RNA (18 to 200 bases) and large RNA (>200 bases) in two separate fractions or total RNA (small and large RNA combined) in a single fraction.
The simple spin-column procedure is said to be convenient and easy to use, allowing RNA and protein isolation without the need for phenol or chloroform.
Highly efficient on-column digestion of genomic DNA provides highly purified RNA samples and each fraction, RNA or protein, is ready to use for further processing or analysis.
For convenience, the Nucleospin miRNA kit is supplied with all that is required to perform the extractions, including a complete set of Nucleospin columns and rDNase.
The kit is suitable for use with a range of tissue types (animal or plant) and cultured cells.
Full protocols for the different fractionation procedures and for different tissue/sample types are described in the user manual provided with each kit.
The Nucleospin miRNA kit is available for 10, 50 or 250 preparations to suit the needs of every laboratory.
