A TTP Labtech study has demonstrated that the Mirroball antibody discovery system offers equivalent data to the discontinued ABI 8200 FMAT instrument, with minimal changes to assay protocols.
Mix-and-read assays, first popularised as FMAT assays, were developed to overcome the problems associated with automating Elisas particularly in cell-based methods.
This comparative study has used the FMAT assay to look at bead-based quantitation of a soluble protein (rabbit IgG) and antibody binding to EGF receptors expressed in A459 cells.
The Mirrorball system scanned the entire well, while FMAT reported data from only a 1mm2 area in each well.
Beads were identified by laser scatter on Mirrorball and fluorescence on FMAT.
The Mirrorball system could enumerate beads in all wells, in contrast to FMAT, which could not detect all beads below 1ng/mL rabbit IgG.
The study demonstrated that the performance of both systems was broadly similar.
For the full application note detailing this comparative study visit the TTP Labtech website.