Softgenetics offers a new version of Nextgene, a freestanding, graphical and interactive Windows-based program for the analysis of short reads generated by massively parallel sequencing platforms.
The latest Nextgene includes a Transcriptome Analysis module for use with short sequence reads generated by Illumina GA/Hiseq, Applied Biosystems's Solid system and Roche Genome GS Titanium and Junior Platforms.
Also included in this release is a Forensic STR Analysis module for use with the Roche platforms.
Kevin LeVan, Nextgene product manager at Softgenetics, said: 'The Transcriptome Analysis module integrated into this version of the Nextgene software is a tool for aligning RNA-Seq data using Softgenetics Indexed Reference Genomes, build 36 or 37 that include multiple annotated transcripts for each gene.
'The software utilises a new algorithm that is better able to align reads spanning exon-exon junctions.
'Reporting is available that identifies the expressed transcripts, as well as novel transcripts and alternate splice variants in addition to the usual variant and expression information of Nextgene.
'The structure variant report generated by the software also includes the sequence of the variant region,' LeVan added.
'We have implemented a novel analysis approach to STR profile analysis utilising longer reads obtained from the Roche Platforms, which enables human identity market participants to utilise the high throughput and lower costs of next-generation sequencing platforms to address the continual backlog of STR profiling endemic to this community,' said Nextgene biologist John McGuigan.
'Furthermore, Nextgene 2.1 now includes a considerable number of additional analysis tools, filtering options, annotation, improved variant call-scoring algorithm, and other functionalities all requested by our customers,' added LeVan.
The company offers 30-day trials and no cost web-based training on its genetic analysis software packages.
