Scientists have used the 3D scanning capabilities of the Quad4 monochromators-based Infinite M1000 microplate reader to investigate nanobodies.
'We have been able to use the 3D scanning capability of the M1000 to study nanobody-induced changes of the spectral properties of GFP in living cells,' said Prof Heinrich Leonhardt, a professor of molecular human biology at Ludwig-Maximilians University Munich (LMU).
'We co-expressed GFP in cells with specific nanobodies that modulate the spectral properties of GFP,' said Dr Carina Frauer, a scientist at LMU.
'The 3D scanning shows excitation and emission on the same plot, illustrating the shift in GFP fluorescence excitation and emission wavelengths induced by nanobody binding,' she added.
