Softgenetics has added a new module to its Nextgene software, version 2.1 and above, that enables the software to process sequence outputs from the Ion Personal Genome Machine made by Ion Torrent.
Developed in collaboration with Ion Torrent researchers as well as Dr Long Phi Le, a molecular pathologist, and Dr John Iafrate, Director of the Diagnostic Molecular Pathology Laboratory, both from Massachusetts General Hospital, the new module converts the sequencing output from the Ion Torrent system into a standard format readable by Nextgene, allowing Ion Torrent data to be used in all the applications supported by Nextgene, including SNP and Indel discovery, capture techniques, RNA-Seq and others.
Nextgene is a free-standing Windows-based software that requires little, if any, bioinformatics assistance, making the software a suitable analysis platform for Ion Torrent data analysis.
Nextgene performs well on low-cost personal computers, processing Ion Torrent capture data in minutes.
The software's Ion Torrent module automatically evaluates the Ion Torrent data, sets analysis parameters, based upon selected application and data input and processing of one or additional projects on a sequential basis with simple point and click operation.
Upon completion, results are presented in a highly graphical integrated interface that allows comprehensive review, sorting, filtering, editing and report printing.
The software also provides confidence scoring of found variants and colour-coded graphical comparison of up to 10 individuals or samples, all of which enable researchers to focus on areas or variants of interest.
