Sigma Life Science has announced the release of Oligoarchitect, an online primer and probe design tool.
This tool enables the automated design of probes and primers for real-time qPCR assays, powered by the industry-standard Beacon Designer platform from Premier Biosoft.
It complements Sigma Life Science's comprehensive portfolio of oligonucleotide and qPCR products and customised oligonucleotide services.
The Oligoarchitect software uses the latest probe design algorithms, providing results in real time and supporting templates of up to 10,000 base pairs.
It also allows users to adjust various input parameters - such as homopolymer run/repeat maximum length, G/C clamp length and maximum primer pair Tm mismatch - to help optimise probe or primer design.
Oligoarchitect currently supports both Sybr Green I and dual-labelled probe (5' nuclease assay) detection chemistries with additional detection chemistries and assays expected to be added later this year, making it suited to applications in traditional PCR, endpoint genotyping, gene-expression analysis, genomic copy number determination, allele discrimination, SNP detection and haplotyping.
