Integrated DNA Technologies (IDT) now offers Primetime Pre-designed qPCR Assays that are guaranteed to work for human, mouse and rat transcriptomes.
Users can search and select from the online database that contains thousands of assays.
All assays are designed to span exons, protect against non-specific amplifications, avoid known SNPs and minimise the impact of secondary structure.
Consisting of two primers and a hydrolysis probe, all three components are shipped in a single tube and are guaranteed to provide greater than 90 per cent efficiency.
IDT's flexibility allows users to select from five different dye/quencher combinations and choose a premixed primer to probe ratio from 1:1 to 4:1, thereby maximising multiplexing capabilities.
The design process uses the most current, clean and complete target sequence information available to generate accurate Tm predictions and protect against off-target amplifications.
Primetime Pre-designed qPCR Assays are quick and easy to order using IDT's unique ordering tool on the company's website.
Each oligo undergoes quality control by mass spectrometry and is shipped within two to four working days.
