GE Healthcare has launched a range of pre-packed gravity-flow columns - rProtein A Gravitrap, Protein G Gravitrap and rProtein A/Protein G Gravitrap - for manual antibody purification.
The gravity-flow columns are said to enable single-step, efficient manual purification of monoclonal and polyclonal antibodies from various species in milligram scale.
The high-capacity columns deliver high-purity purification with a run time of approximately 30 minutes (depending on sample).
GE Healthcare said the columns provide reliable, easy-to-use solutions for small-scale antibody purification.
The product packaging contains everything that is needed for manual purification, as it readily converts into a column stand (workmate) with a plastic tray to collect liquid waste.
Each pack contains 10 pre-packed columns to facilitate multiple samples to be purified simultaneously, using a gravity format.
Columns contain 1ml bed volume and can bind milligram amounts of antibodies.
The columns are reusable up to five times, depending on the sample.
rProtein A Gravitrap contains rProtein A Sepharose FF; Protein G Gravitrap contains Protein G Sepharose 4 FF; rProtein A/Protein G Gravitrap contains 50 per cent rProtein A Sepharose FF and 50 per cent Protein G Sepharose 4 FF.
