Harvard Apparatus offers patented 96-well Dispoequilibrium dialysers, designed to provide a simple and reliable method for simultaneously analysing up to 96 samples on disposable dialysis plates.
These equilibrium dialysis plates are suitable for analysis of free or unbound analytes such as free T4, free Cortisol, free Testosterone, or for Protein-Ligand Binding Studies.
Only available from Harvard Apparatus, these unique, ready-to-use, equilibrium dialysis plates are claimed to be convenient and cost-effective tools for ligand binding experiments, including serum protein binding, protein-drug binding, protein-protein binding and protein-DNA binding assays.
Equilibrium dialysis is widely used in molecular binding studies as it allows for the direct assay of molecular interactions at near-physiological conditions with minimal effects on equilibrium parameters.
Equilibrium Dialysis has also shown itself to be a valuable technique for sample preparation before candidate analysis by mass spectrometry.
Studies have shown enhanced target identification when equilibrium dialysis is used.
Each test-well in the plate consists of two chambers, separated by a regenerated cellulose membrane with a molecular weight cut off of either 5,000 or 10,000 Daltons.
Each chamber holds up to 300ul of sample or buffer.
Dialysis time can be shortened by using a plate rotator, which is available from Harvard Apparatus in a one-, two- or eight-plate formats.
The 96-well plate was designed with an SBS footprint and well spacing to meet automation needs.
Other equilibrium dialysis products from Harvard Apparatus include the single-sample Dispoequilibrium dialyser for volumes up to 100ul; the reusable PTFE Fast Micro-Equilibrium dialyser for samples 25ul to 1,500ul; and the Multi-Equilibrium Dialysis System for up to 20 samples with volumes 200ul to 5ml.
