Integrated DNA Technologies (IDT) offers a complete range of customisable products for RNA interference (RNAi) applications.
The Screening Dicer-substrate siRNA (DsiRNA) is said to provide researchers with a cost-effective and quick solution for their RNAi requirements.
The company's DsiRNAs are chemically synthesised 27-mer RNA duplexes optimised for Dicer processing and incorporation into the RISC complex, resulting in an increase in potency.
IDT's predesigned library includes more than 3,000,000 DsiRNAs designed against human, mouse, rat, cow, dog, chicken, and chimp transcriptomes within Refseq, enabling the production of DsiRNAs to meet the majority of user requirements.
When developing pre-designed DsiRNA duplexes, site selection is first performed using a proprietary algorithm with novel design rules.
The sequences are subsequently screened to minimise the potential for cross-hybridisation and off-target effects, while sequences containing any known small nucleotide polymorphisms (SNPs) or alternatively spliced exons are eliminated.
IDT therefore offers two variants of predesigned DsiRNA duplexes: splice common and splice specific.
Splice common duplexes target all known variants of a gene in Refseq and 8-10 duplexes are available for each target gene.
Splice-specific duplexes target exons present in particular splice variants, making them suitable for researchers targeting specific isoforms.
In addition to the range of pre-designed products, IDT offers researchers custom DsiRNA, RNAi duplex oligos, custom RNAi design as well as antisense oligos.
Produced on IDT's high-throughput synthesis platform, the process has been designed to assure a high-quality product at a low cost.
Custom orders can be placed via the Scitools interface.
