Waters has introduced the Synapt G2-S High Definition Mass Spectrometer, which incorporates high-sensitivity Waters Stepwave ion-transfer optics and Triwave ion-mobility technologies.
It also features a suite of new informatics tools to improve on qualitative and quantitative performance.
Synapt G2-S provides a 30-fold improvement in signal intensity for mass spectral peaks, better than a five-fold improvement in signal to noise and up to a 10-fold improvement in limits of quantitation over previous-generation mass spectrometers.
The significant increase in sensitivity, together with the selectivity and analytical peak capacity made possible by ion-mobility separations allows the Synapt G2-Stoidentify and quantify sample analytes at lower concentrations than any other high-resolution mass spectrometer and easily and routinely reveal details about complex samples that were previously difficult or impossible to obtain.
Stepwave ion-transfer optics technology employs an off-axis design for dramatically increasing the efficiency of ion transfer from the ion source to the MS analyser, while at the same time actively eliminating undesirable neutral contaminants.
The resulting sensitivity improvement is critical, for example in metabolite identification where the goal is to find, identify and quantify all metabolites in a sample.
Experiments such as these can be performed for the first time at biologically relevant concentration levels, which give the chemist a much more accurate quantitative and qualitative understanding of the molecular system of interest to better aid drug-development processes.
Waters' Synapt G2-S supports Waters' High-Definition MS(E) acquisition method, which comprehensively catalogues complex samples in a single analysis by separating components by ion mobility and then capturing the molecular and fragment ion data from all detectable peaks across the entire chromatographic separation - all in a single injection.
The ability of ion mobility to separate isobaric/isomeric compounds from one another is the key to reaping the benefits of the sensitivity enhancements of Stepwave, dramatically increasing the number of confidently identified components.
The result is accurate qualitative and quantitative profiling of all of the components over a linear dynamic range of up to five orders of magnitude aided by the high-resolution quantitative time-of-flight (Quantof) analyser featuring an ultra-fast electron multiplier and hybrid analogue-to-digital (ADC) detector electronics.
The instrument's mass resolving power is more than 40,000FWHM (full width half maximum).
Synapt G2-S also includes additional software to provide experimental versatility to meet a range of challenges: MS(E)data viewer for visualisation, processing and interpretation of multidimensional MS or HDMS data; high-definition imaging software for leveraging the ability of ion-mobility separations to maximise selectivity and confidence in Maldi imaging experiments; dedicated proteomics and biopharmaceutical software application managers that fully support HDMS(E) data processing and reporting.
Improved features allow scientists to take advantage of Synapt's Time-Aligned-Parallel fragmentation capability and Electron Transfer Dissociation (ETD) capability - providing faster, more complete and confident structural identification and characterisation of target analytes.
Combining faster spectral acquisition rates of up to 30 spectra/sec with new embedded algorithms to optimise data-dependent analyses (DDA) to efficiently deliver high-quality MS/MS spectra for compound identification or protein quantification by isotope labelling.
The Synapt G2-S also features Waters' Universal Ion Source Architecture, which is engineered to take maximum advantage of UPLC and allow the widest range of Waters' ionisation sources, including: electrospray (ESI), atmospheric pressure chemical ionisation (APCI), dual ESI and APCi (ASCi), atmospheric pressure photoionisation (APPI), atmospheric pressure gas chromatography (APGC), Nanoflow (ESI), matrix-assisted laser desorption (MALDI), atmospheric solids analysis probe (ASAP) and Trizaic UPLC.
It is also compatible with third-party ionisation sources including DESI (Prosalia), DART (Ionsense), LDTD (Phytronix) and the Triversanano Mate (Advion) sources.
Primary applications for the Synapt G2-S include proteomics, biomarker discovery, pharmaceuticals/biopharmaceuticals, lipidomics, structural elucidation, metabolomics, polymer analysis, petroleum characterisation, metabolite identification and food research.
