Sartorius Stedim Biotech has launched a single-use bioreactor with a two-litre working volume, suitable for mammalian, insect and plant cell culture tasks in research, development and production.
The Univessel SU is designed for applications ranging from small-scale protein expression to media or process optimisation studies.
The Univessel SU is irradiated prior to delivery and is ready to use out of the box.
It is designed to the same safety standards that are required for classical multi-use bioreactors.
The complete culture vessel is disposed of after use, thus reducing cleaning time and eliminating autoclaving and re-installation hassle as well as the risk of cross contamination.
The single-use vessel can be connected to all Sartorius Biostat bench-top bioreactor controllers as well as other controllers for conventional glass reactors.
When working with existing systems, classical pH and DO sensors as well as Pt100 temperature probes can be inserted via top plate ports.
Made of USP class VI conform polycarbonate, the Univessel SU includes several ports for media addition, sampling, harvesting and spare ports for inserting classical sensors or other equipment suitable for pg 13,5 ports.
Three-blade segment impellers and a L-sparger provide optimal conditions for cell culture applications.
The torospherical bottom and the height-diameter ratio of the Univessel SU are similar to the design of the classical glass Univessel.
All tubings for media transfer are made of thermoweldable tubing and feature common Luer or MPC connectors for connection to peripheral equipment.
The vessel comes with a secure stand as well as a stainless steel vessel holder to provide a safe support stand when the vessel is in operation.
Midisart BV air filters for sparger and overlay inlet and the gas outlet provide sterile operation conditions over a long period of time.
Temperature control is performed by a heating blanket and a filter heater is included to ensure long operation times of the exhaust filter.
Single-use sensors for the optical measurement of pH and dissolved oxygen (DO) are available and enable safe and contamination-free measurement together with the Biostat B-DCU II bench-top controller.
All single-use sensors are pre-calibrated.
The calibration information is displayed as a barcode on the vessel label and can be transferred via a barcode reader directly into the Biostat B-DCU II controller.
Already used in other single-use cell culture devices by Sartorius Stedim Biotech, this principle of non-invasive pH and DO measurement is now also available with small-scale single-use bioreactors.
