Fluidigm Corporation has announced the availability of the Access Array Barcode Library for the Ion Torrent Personal Genome Machine (PGM) sequencer
According to the company, the barcode library allows multiplexing of up to 96 different samples in a single sequencing run.
"The library uses a bidirectional amplicon tagging approach to allow reads to be obtained from both ends and across the full length of the amplicon with single-read sequencing.
"This eliminates the need for paired-end read sequencing," said Gajus Worthington, Fluidigm President and CEO.
The Fluidigm Access Array System prepares amplicons for resequencing without the need for any additional library preparation and can operate with any next-generation sequencer (NGS) on the market, said the group.
It prepares amplicons in hours and performs the sample preparation for around $10 per sample.
In addition, using the Access Array Barcode Libraries developed for the Illumina, Roche, and Ion Torrent sequencing platforms, researchers can test the same amplicon pools to cross-validate sequencing results across different NGS platforms.