Biotech Support Group reports on the use of Albusorb to remove albumin from rat serum samples.
The use of Albusorb was part of a study to determine apoCIII’s increased levels in serum in type 1 diabetes patients.
Apolipoprotein C-III is an important protein component of triglyceride rich lipoproteins and HDL.
The glycoprotein Apolipoprotein C-III (apoCIII) inhibits lipolysis and is documented to play a vital role in the development of hypertriglyceridemia when increased.
During the study Albusorb from Biotech Support Group was used to remove albumin from rat serum samples.
Scientists setup experiments implementing the animal model diabetes-prone BB rat (DPBB) to ascertain if apoCIII increases contributed to calcium increase and B-cell death in vivo.
Albusorb is an albumin depletion reagent supplied as a kit with necessary buffers.
It comes from a silica-based, separation platform utilising a new combination of surface microenvironments substituted with low molecular substrates that feature drug-binding motifs.
Features overview:
- Removes >90% albumin from 30 mg albumin/ml sample
- Affinity-type equivalence, virtually no cross-reactivity with other proteins
- Bind and elute procedure - simply weigh powder, condition the sample, centrifuge and/or filter, and recover the albumin depleted serum
- Economical new surface technology, not based on affinity chromatography
- Mild conditions maintain tertiary structure of proteins and simple transfer to secondary analysis
- The albumin depleted filtrate retains the enzymatic and biological activity
- Removes albumin from samples such as serum, plasma and from species including human, mouse, sheep, bovine, goat, rat, and calf
- The flow through fraction is compatible with LC-MS, activity based protein profiling and proteomic studies.
To download the full report, please click on the link above.
