Latest three of six new microarrays for high-sensitivity gene expression research this year offer improvements in detection and reproducibility
Agilent Technologies Europe has introduced three industry-first, 60-mer catalogue oligo microarray kits.
The microarray kits, used by researchers in disease research and drug discovery, demonstrate significant improvements in sensitivity over the 25-mer probe length format and are based on industry-standard 1"x3" glass slide formats, which make them easy to integrate into established microarray laboratories.
The addition of these microarrays brings Agilent's total of new microarray kits released this year to six.
Agilent's new 60-mer microarray format offers researchers and scientists improvements in sensitivity with the ability to detect one transcript per cell per million cells, and the ability to display reproducible data with a log ratio mean standard deviation of 0.015.
"We are committed to offering our customers a wide variety of high-quality catalogue microarrays, and with this release our customers are now able to employ either our existing cDNA or our new 60-mer oligo microarray formats," said Barney Saunders, vice president and general manager of Agilent's BioResearch Solutions unit.
"These new microarray kits have been shown to deliver remarkable improvements in sensitivity, are based on industry standards, and include unique content and access to sequence information and annotation." The rapid introduction of new Agilent microarrays has been enabled by the company's proprietary ink-jet style SurePrint manufacturing process, which has been refined to print high-quality, highly consistent cDNA and oligo microarrays in a very short period of time.
The 60-mer oligo design has been available for over a year to researchers through Agilent's custom microarray programme and has demonstrated significant improvements in sensitivity.
Agilent is now using its 60-mer printing capability to build a new series of ready-to-use catalogue microarrays with the following characteristics: High sensitivity - Agilent's new 60-mer microarray format has been shown to deliver improvements in sensitivity with the ability to detect one transcript per cell per million cells.
High reproducibility - precision printed catalogus microarrays provide reproducible data with a log ratio mean standard deviation of 0.015.
Quality data - lowered noise levels yields more consistent data and better detection.
Access to sequence information - 60-mer probe sequence and annotation information is available to all users.
Comprehensive content - all catalogue oligo microarrays feature the most current publicly available content at the time of product introduction.
Customisation - Agilent's flexible on-demand printing process provides the ability to easily revise, customise and add new content.
The first release in this series of new catalogue 60-mer oligo microarrays includes human, yeast and mouse development microarray kits.
Each kit includes microarrays pre-printed on barcoded glass slides for easy sample tracking.
Human 1A oligo microarray kit - based on the latest content from Incyte Genomics, this new microarray contains an experimentally validated set of 60-mer probes for screening 17,803 full-length genes.
Complete 60-mer probe sequence information and enhanced protein annotation are made available for every gene sequence. Mouse development oligo microarray kit - this microarray offers more than 20,000 60-mer probes representing genes from the well known 15K clone set and the newly released, non-overlapping 7.4K clone set.
A full 98% of the probes correspond to clones in this publicly available collection.
Yeast oligo microarray kit -this microarray provides more than 10,000 60-mer probes representing 6,256 known open reading frames (ORFs) in the yeast genome.
For enhanced performance of Agilent microarrays, Agilent is also offering a series of labelling and hybridisation kits and accessories.
