A decreased cost of membrane array experiments with increased number of targets detected are promised by optimised hybridisation reagent and conditions
Ambion's Array-Advantage probe labelling and hybridisation kits increase the value of membrane arrays by allowing them to be stripped and reprobed 10 times or more without loss of signal. Kits contain all necessary components for probe labeling by reverse transcription, membrane hybridisation, and probe stripping, except for label.
Reagents and protocols for the Array-Advantage Kits are specifically designed to work with Clontech's Atlas Array (the AA Kit), Research Genetics' GeneFilters Array (the GF Kit), and Ambion's UltrArray or Incyte Genomic's LifeGrid Array (the UA/LG Kit).
The value of commercial membrane arrays is extended dramatically when used with the Array-Advantage kits.
These feature Ambion's Strip-EZ Technology, which involves reverse transcription of a probe that is stable under conditions used for hybridisation and washing, but can be degraded after detection.
Mild wash conditions preserve the nucleic acid targets bound to the blot. The Array-Advantage Kits also contain UltrArray Membrane Hybridisation Buffer and wash buffers that consistently provide the greatest sensitivity by maximizing signal and minimizing background.
