The Poly(A) Tailing Kit uses E.coli Poly(A) Polymerase I to polyadenylate the 3' termini of in vitro transcribed RNA transcripts.
The Poly(A) Tailing Kit uses E.coli Poly(A) Polymerase I to polyadenylate the 3' termini of in vitro transcribed RNA transcripts.
Polyadenylation plays an important role in the stabilization of RNA transcripts in eukaryotes and enhances the efficiency of translation in vivo.
Polyadenylation of capped RNA transcripts has been shown to increase protein synthesis 5 - 10 fold.
The Poly(A) Tailing Kit is an adjustable reaction that adds a desired tail length without prior purification of transcribed RNA.
* Adds a poly(A) tail of at least 150 nucleotides in length. * Enhances translational efficiency in vivo.
* Reaction can be controlled for tail length.
