The new Cells-to-Signal kit from Ambion uses a chemical lysis method to create cell lysates in less than five minutes at room temperature
The cell lysate can be used directly for RT-PCR, bypassing RNA isolation altogether.
Cells-to-Signal is said to be ideal for monitoring siRNA-induced knockdown.
The Cells-to-Signal procedure is highly reproducible, compatible with a wide variety of cell types, and offers a single-step procedure that is optimised for high throughput analyses platforms.
