Can detect and measure up to 400 antibody binding interactions simultaneously on one chip in under two hours, accelerating analysis up to 100 times faster than existing technologies
Applied Biosystems has recently introduced the 8500 Affinity chip analyser, a system for rapidly identifying and characterising potential antibody, diagnostic and therapeutic candidates.
The 8500 system can detect and measure up to 400 antibody binding interactions simultaneously on one chip in under two hours, accelerating analysis up to 100 times faster than existing technologies on the market.
The instrument uses surface plasmon resonance (SPR) to measure the binding of biomolecules in real time without using fluorescent dyes or radioactivity.
The 8500 system format allows researchers to directly compare binding data within the same experiment, reducing run-to-run experimental variability while increasing throughput.
The primary applications of the 8500 system include antibody affinity determination, identification of matched antibody pairs and peptide epitope mapping.
Additional applications are under development, such as the characterisation and identification of protein-protein and protein-nucleic acid interactions.
Applied Biosystems plans to introduce proteomic-based applications during 2004.
The 8500 Affinity chip analyser is a complete system with associated reagents and consumables, and it complements the Applied Biosystems 8200 cellular detection system, which is used for primary antibody screening applications and other cell- and bead-based functional assays.
