Formulation gives high performance with cDNA as well as genomic and plasmid DNA templates over a broad dynamic range, achieving sensitive and specific amplification
The iTaq Supermix with Rox is a new real-time PCR supermix designed for probe-based detection on all Rox-dependent optical thermal cyclers.
The formulation gives high performance with cDNA as well as genomic and plasmid DNA templates over a broad dynamic range, achieving sensitive and specific amplification over at least seven orders of magnitude, says Bio-Rad The supermix is pre-blended with hot-start iTaq DNA polymerase, optimised buffer, nucleotides including dUTP, and the passive reference dye Rox. Its convenient one-tube formulation enables users to obtain sensitive and specific amplification every time.
Features of this novel formulation of real-time PCR include the following:. Contains the passive reference dye Rox for normalisation of fluorescent signals and well-to-well optical variations.
All reaction components are pre-blended for ease of use on all Rox-dependent platforms and easy set-up and storage.
Unique low foam formulation minimises bubble formation during pipetting, thereby improving accuracy.
Contains hot-start iTaq DNA polymerase for stringent PCR amplification.
Supermix incorporates dUTP for optional UNG pre-treatment of possible contaminants.
