Two new product ranges, designed specifically for use in flow cytometry, offer multiplex beads for solution array analysis and microsphere standards kits for flow cytometry
Two new product ranges, designed specifically for use in flow cytometry, have been introduced by Duke Scientific Corporation. Brookhaven International is authorised distributors for these and other Duke Scientific products.
Cyto-Plex: multiplex beads for solution array analysis.
High throughput and the ability to detect and quantify multiple analytes simultaneously are the hallmarks of solution array technology.
New Cyto-Plex microspheres provide colour-coded microspheres that enable the use of these array techniques in flow cytometry.
Each kit consists of up to ten levels of red fluorescent dyed beads that are excited by either 488nm (argon) or 633nm (He-Ne) lasers.
The microspheres emit strongly in the FL3 and FL4 channels with minimal emissions in FL1 and FL2.
Multi-analyte experiments can be designed utilising either FL1 or FL2 probes.
These beads exhibit very low non-specific binding and have hydrophilic surface-active carboxyl groups for conjugation to antibodies, nucleic acids and other biomolecules.
Cyto-Cal: microsphere standards kits for flow cytometry.
The Cyto-Cal range consists of three distinct but complementary products aimed at optimising linearity, optical performance and sensitivity.
Optimise instrument linearity and range with Intensity Standards Kits. These highly uniform intensity calibration microspheres are specifically designed for simultaneously establishing both the sensitivity and range of a flow cytometer.
The calibrator beads with the two lowest intensities simulate weakly labelled and autofluorescent cells, allowing the instrument's sensitivity to be maximised.
Five different fluorescence levels in each vial provide a full range of intensities calibrated with approximate MESF values to determine the relative fluorescence of labelled cells.
Intensity Standards Kits are available for fluorescence channels FL1, FL2 and FL3 (corresponding to 530, 575, and 700nm respectively).
Optimise instrument performance with Cyto-Cal 488 and 633 Alignment Standards Kits.
Exceptional precision in size and fluorescence uniformity make these 3-micrometre alignment standard microspheres ideal for optimising the instrument's optical detection parameters.
Knowing that the flow properties and focusing optics are tuned to their full potential greatly increases confidence in the data collected.
Optimise instrument sensitivity with Low Intensity Standards Kit.
Discrimination of weakly fluorescent labelled cells from background and instrument noise is critical for many applications.
Duke's Low Intensity Standards Kit consists of 6 micrometre beads with broad-range fluorescence together with unlabelled beads and is designed to simulate a mixture of dim labelled cells and unlabelled cells.
For the ultimate in sensitivity, this kit allows the flow cytometrist to monitor the FL1, FL2 and FL3 channels simultaneously for consistent intensity-range calibration at low levels of fluorescence.
