Chromogenic medium will clearly differentiate L monocytogenes from L ivanovii and other listeria species by a simple colour change reaction within 24 hours
Rapid L mono agar, developed by Bio-Rad Laboratories, is a chromogenic medium that will clearly differentiate L monocytogenes from L ivanovii and other listeria species by a simple colour change reaction, within 24 hours of pre-enrichment. Identification is based on the specific detection of phosphatidylinositol phospholipase C (PIPLC) activity, resulting in a blue colony, and the inability of L monocytogenes to metabolise xylose, resulting in the absence of a yellow halo. Rapid L mono agar has been approved by Afnor, AOAC, MFHPB (Canada) and has been submitted for NordVal approval.
This chromogenic medium is also recommended by FDA/BAM.
