Novel pFIVT siRNA lentiviral cloning and expression vectors efficiently introduce and express siRNA in any mammalian cell including dividing and quiescent cells
Cambridge BioScience has been appointed distributor for System Biosciences in the UK and Ireland.
Through the development of unique and innovative technologies, System Biosciences provides researchers with the necessary tools for investigating genomic and proteomic function.
Novel pFIVT siRNA lentiviral cloning and expression vectors efficiently introduce and express siRNA in any mammalian cell including dividing and quiescent cells.
This makes it possible to study difficult-to-transfect cells such as primary cultures, stem cells, neurons, endothelial cells and retinoblastomas.
The pFIV siRNA cloning system permanently silences genes allowing the user to set up a long-term siRNA knockdown system or to generate gene-specific knockdown animals such as transgenic mice.
As the most efficient way to deliver siRNA constructs, lentiviral transduction minimises the side effects produced by harsh and disruptive transfection protocols. The combination of siRNA and effector expression library technologies enables the user to conveniently introduce and study the effect of many thousands of siRNA molecules on cell functions at once using lentiviral vector-mediated transduction.
After selecting transduced cells that are expressing the desired phenotype, the sets of genes controlling a biological response can be easily identified.
These products are available from the UK and Ireland only.
