Until now, expanding naive and antigen-specific mouse CD4+ and CD8+ T cells has involved tedious and unreliable extraction and maintenance of antigen presenting cell cultures
Research into many human diseases will be made easier by the release of an innovative new way to expand mouse T cells without autologous antigen-presenting cells and antigen, says Dynal Biotech.
Researchers begin work with mouse models before progressing to human disease models, including the study of infectious diseases, autoimmunity and cancer.
Expanded mouse T cells are manipulated in vitro or used for adoptive transfer in vivo.
Until now, expanding naive and antigen-specific mouse CD4+ and CD8+ T cells has involved tedious and unreliable extraction and maintenance of antigen presenting cell (APC) cultures. Dynabeads Mouse CD3/CD28 T Cell Expander is the first ready-to-use system to provide simultaneous signals to TCR/CD3 and CD28 for full activation and expansion of mouse T cells in culture.
This mimics in vivo stimulation of T cells by APCs. These Dynabeads are coupled with antibodies against CD3 and CD28 in optimised ratios to imitate in vivo stimulation by antigen-presenting cells.
The antibodies are covalently coupled onto cell-sized beads, giving a stable product with consistent stimulation and expansion capabilities.
This new, easy-to-use product complements an existing Dynal product for expanding human cells, bridging the gap from mouse to human models without having to worry about maintaining APCs in culture.
