Various kinds of ligations with blunt or sticky end DNA fragments as well as T/A overhangs can be performed in a minimum time - in most cases, one minute reaction time is enough
The Genaxxon Alligator DNA ligation kit is a simple two component system that allows DNA ligation reactions to be performed very rapidly.
The kit uses T4 DNA ligase and an optimised proprietary buffer system.
Due to the extremely high efficiency of the ligation reaction, conventional overnight incubations are said to be no longer necessary.
Various kinds of ligations with blunt or sticky end DNA fragments as well as T/A overhangs can be performed in a minimum time - in most cases, one minute reaction time leads to sufficient efficiency.
The reaction can proceed directly to bacterial transformation.
By using the Genaxxon competent cells shipped with Alligator the whole ligation and transformation procedure can be done within 20 minutes.
Alligator can be purchased in package sizes to perform 30 reactions up to 120 reactions.
