Technical application bulletin focuses on how using conventional evaporation techniques to dry water / organic solvent mixtures from LC/MS factions can prove problematic
Available now from Genevac is a technical application bulletin that discusses the important issues for scientists seeking to optimise solvent evaporation from LC/MS fractions.
The four page bulletin focuses on how using conventional evaporation techniques to dry water / organic solvent mixtures from LC/MS factions can prove problematic.
Three main problems are highlighted and the technological solutions adopted by Genevac to overcome them discussed.
The bulletin demonstrates the utility of the Genevac Dri-Pure system in preventing the phenomenon of 'bumping'.
Results illustrate how Dri-Pure can be used to suppress the ejection of superheated solvent and solute during evaporation that can lead to sample loss and cross-contamination. The varying rate of evaporation of LC/MS fractions can also have a negative effect on the efficiency of rapid solvent evaporation using infra-red heat.
Using Genevac's unique SampleGuard temperature control system the benefits of being able to set a temperature ceiling for sample holders are illustrated.
This is of special importance to thermolabile samples (those that contain less water) that evaporate to dryness and thus potentially may be exposed to high levels of heat once dry.
Finally the bulletin reviews the problem of rotor imbalance caused by some LC/MS fractions evaporating faster than others during centrifugal evaporation.
The bulletin discusses how Genevac's Auto-balancing mechanism can increase the productivity of the evaporation process by eliminating the need to carefully balance samples prior and during the solvent removal process.
