Laboratories producing monoclonal antibodies for numerous biological applications using tissue culture flasks or roller bottles are set to benefit from this development
As easy to use as tissue culture flasks or roller bottles, the Celline cultivation system from Integra Biosciences offers significant benefits to laboratories involved with monoclonal antibody (mAb) production.
Using the Celline system, concentrations of 1 to 4mg/ml leading to yields of 200mg/month with a single bioreactor are routinely achievable.
This equates to over 100 x 175ml culture flasks run in batch mode.
Celline, with its novel membrane technology, enables higher antibody concentrations to be used in the cultivation chamber, highly concentrated mAb products to be obtained, and physiologically optimised cultivation conditions to be achieved.
Compared to traditional bioreactors or tissue culture flasks stacked in incubators, Celline significantly reduces material use, working costs and the time taken to harvest high concentrations of monoclonal antibodies.
The result is a typical cost reduction of 40%.
Designed for ease-of-use, Celline utilises proprietary membrane technology to separate off the cultivation chamber with an upper semi-permeable membrane through which nutrient can diffuse and a lower one that allows gases to diffuse.
Separate ports allow selective access to the upper nutrient supply chamber and the central cultivation chamber.
This novel compartmentalised arrangement means that medium can be exchanged without influencing the function or growth of the cells and gases. Consequently, traditional antibody growth limitations brought about by a lack of nutrients or the accumulation of metabolic waste can be overcome just as easily as oxygen deficiency.
Such optimised conditions promote significant cell growth and lead to drastically increased antibody densities even to the extent of 3-dimensional growth.
