System uses an enzymatic background reduction method that enhances reliability and facilitates complete automation of the genotyping process
Promega's new Readit version 1.1 SNP genotyping system uses an enzymatic background reduction method that enhances reliability and facilitates complete automation of the genotyping process.
Equally suitable for laboratories with low and high throughput requirements, the new method enables analysis in a manual 96 well format or automated analysis in 96 and 384 well formats.
Readit technology detects specific alleles by combining novel enzymatic reactions and sequence specific oligonucleotide hybridisation.
Versatile and flexible for the detection of sequence variation or genotype, the assay combines enzymatic fidelity, stringent probe hybridisation and an integrated data calculator for automated SNP scoring, error detection and statistical analysis of the data.
Providing increased confidence in results, validation studies reveal a genotyping accuracy of greater than 99.9%.
The Readit system is suitable for the detection of SNPs, estimation of allelic frequencies and allele correlation studies, the detection of chromosomal translocations, mutation detection and the interrogation of PCR products to determine the presence of a known sequence.
Readit version 1.1 allows users to design their assays both in terms of the target and their throughput requirements.
