One-step assay for reproducible results in high throughput laboratories provides a stable luminescent signal at room temperature that can be measured from 30 minutes to four hours
Enabling high throughput of several thousand samples per hour, Promega's new one-step Beta-Glo assay system for the quantitative measurement of beta-galactosidase expression in mammalian cells, provides a stable luminescent signal at room temperature that can be measured from 30 minutes to more than four hours after reagent addition.
The extended luminescence signal, which is directly proportional to the amount of beta-galactosidase in the sample, eliminates the need for reagent injectors and provides flexibility for the batch or continuous processing of multiple plates.
Extremely sensitive and with a wide dynamic range, Beta-Glo can detect 100 femtograms of beta-galactosidase with a linear range extending over four logs of enzyme concentration.
Researchers can obtain reliable and reproducible results in both low-volume formats or in samples with low-level expression.
Simple and convenient to use, the homogeneous assay requires the addition of just one reagent directly to cells in culture in 96- or 384-well plates, without any need for prior sample processing.
Results can be read with any luminometer and injectors are not required.
Researchers, in both traditional and high throughput environments will find Beta-Glo offers clear benefits in terms of convenience and brightness of the luminescence signal.
