Vectors contain degradation sequences that destabilise the luciferase enzyme and lower the accumulated intracellular pool, more quicker and more sensitive response
It is now possible to quantitate smaller relative changes in gene expression by using the Rapid Response reporter vectors from Promega.
Greater sensitivity is achieved due to a faster reporter response to stimulus, and assay incubation times are also significantly reduced by up to 75%.
These two factors combine to reduce the risk of secondary effects (eg cytotoxicity) and improve screening efficiency.
The vectors contain degradation sequences that destabilise the luciferase enzyme and lower the accumulated intracellular pool.
The resulting reporter proteins therefore have half-lives that are 60% less than more stable equivalents.
This serves to dramatically improve the temporal coupling (or time delay) between transcription and reporter signal.
Consequently much earlier measurement of both up- and down-regulation upon stimulation is possible.
Non-specific activation of the Rapid Response reporter vectors is minimised because these genes have been designed to be free of most known consensus sequence transcription factor binding sites.
Codon usage has also been optimised for mammalian systems to enhance translation efficiency.
Complete compatibility with Promega's industry standard luminescence assay reagents enables the most rapid, sensitive and reliable reporter gene measurements.
Rapid Response reporter vectors are available optimised for either Renilla or firefly luciferases.
For each of these formats, there are two configurations that contain protein and mRNA degradation sequences to provide the fastest and greatest magnitude of response, or protein degradation sequences only which produce higher total levels of expression, together with faster response rates.
