Functionally validated primer-probe sets for quantitative real-time PCR are available for both the human and the corresponding mouse sequence
New QuantiTect gene expression assays are functionally validated primer-probe sets for quantitative real-time PCR - delivered ready to use in a master-mix format.
The high efficiency of QuantiTect probe kits in one- and two-step RT-PCR combines with extensive validation of assays to guarantee optimal results on any real-time thermal cycler.
QuantiTect gene expression assays are available for both the human and the corresponding mouse sequence.
Qiagen regularly updates a range of validated QuantiTect gene expression assays, starting with assays for cytokine, chemokine, and housekeeping genes.
QuantiTect custom assays can be easily designed for any known gene at the Qiagen website.
All assays are validated for PCR efficiency and sensitivity in both one-step and two-step RT-PCR on a range of real-time cyclers, ensuring accurate results and flexibility in experimental design.
In contrast to other commercial assays, primers are designed to anneal to exon/exon boundaries.
This prevents co-amplification of genomic DNA, which can compromise assay sensitivity and efficiency.
