Two new bead-based assay kits facilitate research in drug discovery, cell signalling, oncogenesis, apoptosis, and immune disorders
Two new LiquiChip bead-based Xmap assay kits from Qiagen facilitate research in drug discovery, cell signalling, oncogenesis, apoptosis, and immune disorders.
Optimised immobilised substrates enable detection of a wide range of Tyr and Ser/Thr kinase activities without the need for radioactivity. The ready-to-run kits contain everything needed for fast and accurate assay and detection.
In LiquiChip broad-range kinase kits, peptides or proteins that serve as Ser/Thr or Tyr kinase substrates are immobilised on LiquiChip beads.
Substrate-coated beads are incubated with a sample containing one or more active kinases and a non-radioactive Mg2+-ATP cocktail that serves as a phosphate donor.
The effect of potential or known inhibitors on kinases can also be easily investigated.
After the reaction, phosphorylation sites are detected by primary antibodies that specifically recognise phosphorylated serine, threonine, or tyrosine residues.
Primary antibodies are detected using a biotinylated secondary anti-mouse antibody.
Secondary antibodies are detected in turn using streptavidin conjugated to highly fluorescent phycoerythrin.
Reversible protein phosphorylation has been known for some years to control a wide range of cellular processes and activities such as transmembrane signaling, intracellular amplification of signals, and cell-cycle control. LiquiChip broad-range Ser/Thr and Tyr kinase kits can be used for sensitive measurement of a wide range of kinase activities in cell signalling and cellular pathway studies, drug screening, and kinase inhibitor characterisation.
