A uniform yield of amplified DNA is usually achieved regardless of the quantity of template DNA, enabling immediate downstream genetic analysis by conventional techniques
Qiagen says its Repli-G kit and service provide highly uniform DNA amplification across the entire genome, with minimal sequence bias.
Various clinical and non-clinical research samples can be used, including genomic DNA, fresh or dried blood, buffy coat, and tissue culture cells.
Typical DNA yields from a Repli-G kit reaction are approximately 40ug per 50ul reaction.
Qiagen says that a uniform yield of amplified DNA is usually achieved, regardless of the quantity of template DNA, enabling immediate downstream genetic analysis, including SNP genotyping, STR analysis, Southern blotting and RFLP analysis, subcloning, and DNA sequencing.
The Repli-G service provides amplification of unlimited amounts of DNA from limited samples.
A stringent quality control assay provides information on the quality of the amplified DNA, enabling reliable predictions for the success of your downstream assay to be made.
Repli-G technology is based on multiple displacement amplification, which carries out isothermal genome amplification utilising a uniquely processive DNA polymerase capable of replicating 100kb without dissociating from the genomic DNA template.
The DNA polymerase has a 3'-5' exonuclease proofreading activity to maintain high fidelity during replication.
