Because this plate allows for simultaneous cell lysis and protein capture, it will reduce the screening time of 96 recombinant clones for protein production from an entire day to two hours
Sigma has launched a new His-Select Ilap high capacity 96-well plate (product code H 9412).
The Ilap (integrated lysis and affinity purification) plate is a patent-pending product that combines the cell lysis and His-tagged protein purification steps into one.
This exclusive new plate reduces the number of steps and offers substantial time saving for high throughput purification of His-tagged proteins.
In addition, the His-Select technology of the plate provides high specificity and binding capacity for histidine containing fusion proteins.
The plates are coated with both a cell lysis reagent and the His-Select nickel chelate matrix.
This technology allows for cells to be lysed and the His-tagged protein to be captured all in one step.
Cell lysis and protein extraction is highly efficient and eliminates the need to harvest cells from the culture.
The His-Select coating is highly selective for His-tagged proteins, reduces non-specific binding, and has a high binding capacity.
"The His-Select Ilap plate has dramatically simplified high-throughput purification of His-tagged proteins," commented Michael Hadjisavis, manager of strategic business, protein expression and proteomics, at Sigma-Aldrich.
"Because this plate allows for simultaneous cell lysis and protein capture, it will significantly reduce the screening time of 96 recombinant clones for protein production from an entire day to two hours.
"It will also simplify other high throughput applications for characterising His-tagged proteins like protein:protein interaction assays."
