Multi-functional image analysis system can be used to detect and analyse sensitive protein stains, providing a new way to automate gel-based proteomics
Syngene says that its ChemiGenius2 multi-functional image analysis system can be used to detect and analyse the sensitive protein stains, Deep Purple and Sypro Ruby.
This will benefit users looking for an affordable, time saving method of automating their gel-based proteomics studies.
Imaging Deep Purple and Sypro Ruby fluorescent dyes is possible with a ChemiGenius2 because its high performance camera detects a wide range of dyes that have UV excitation peaks.
Additionally, since the ChemiGenius2 darkroom is fitted with a filter wheel, white light pad with optional dual wavelength transilluminator, as well as overhead white and epi-UV light, it is easy to achieve the right illumination conditions for these and many other protein and DNA dyes.
After careful research, Syngene's technical team found using a long wave transilluminator and UV filter produced optimum results for Deep Purple stained gels.
For those stained with Sypro Ruby, a medium wave transilluminator and UV filter or medium wave transilluminator with a Syngene blue light converter and Syngene SG03 filter generated the best images.
The commonly used method for detecting Sypro Ruby and Deep Purple stained proteins is laser-based scanners but these are expensive and many cannot detect traditionally used visible proteomic stains, such as Coomassie Blue or Silver.
However, by using the ChemiGenius2 with a white light pad and Syngene neutral fielding correction, the system can image both of these dyes with ease.
Laura Sullivan, Syngene's divisional manager, said: "We are excited that the ChemiGenius2 can rapidly detect more sensitive fluorescent protein dyes, as well as standard visible ones. "Its versatility makes the ChemiGenius2 an excellent, cost-effective alternative to laser-based scanners and will prove invaluable for a range of proteomics gel applications."