Specific removal of over 95% human albumin from a human sample is achieved while no significant amounts of other serum or plasma proteins are bound
Vivascience introduces new Vivapure Anti-HSA kit for human albumin depletion.
The new Vivapure kit is based on new and unique Anti-HSA antibody fragments coupled to cross-linked agarose for achieving high albumin binding capacities.
In contrast to traditional dye based ligands, specific removal of over 95% human albumin from a human sample is achieved while no significant amounts of other serum or plasma proteins are bound. This procedure improves the resolution and detection of lower abundance sample proteins by electrophoresis, chromatography and mass spectrometry.
Though the Anti-HSA ligand has been specifically produced against human serum albumin, some reactivity remains against rat and mouse albumin so that albumin removal rates of 50-80% are reached for samples from these organisms.
The new Vivapure Anti-HSA kit for human albumin depletion is the preferred product used for human serum preparation at Eurogentec before proteome analysis using high resolution 2D Page.
The Vivapure Anti-HSA kit for human albumin depletion contains Anti-HSA affinity-resin, centrifugal columns which can individually be filled with the Anti-HSA resin amount necessary, micro-centrifuge tubes as well as binding buffer for preparing 12 serum samples.
The spin column format allows the processing of a single sample within 20 minutes and is just as convenient for the parallel processing of several samples simultaneously using standard laboratory equipment.
