Integrating field flow fractionation with multi-angle light scattering detection means that reproducible absolute particle sizing can be achieved without calibration or sizing standards
Wyatt Technology reveals what it says is the perfect duo - the Eclipse and the Dawn EOS - to make absolute particle sizing an accessible alternative to traditional liquid chromatography methods.
Individually, both instruments offer state-of-the-art functionality, but when combined, they form the ultimate package for the biotechnology and pharmaceutical applications.
By integrating the Eclipse field flow fractionation with the Dawn multi-angle light scattering detection, reproducible absolute particle sizing can be achieved without the need for calibration or sizing standards.
The Dawn EOS is said to be the world's most advanced commercial instrument for absolute macromolecular characterisation.
Wyatt says it is the only multi-angle light scattering (Mals) detector capable of determining radius and molar mass results simultaneously, and is able to calculate masses directly from the variation of the light scattering pattern.
It works with absolute precision, meaning that there is no need to refer to standards or assumptions a priori.
Due to its versatility, the Eclipse particle fractionation device, plays a significant role in a wide range of research and development, including molecular biology, nanotechnology and environmental analysis.
It produces extraordinary-yet extraordinarily gentle-particle and molecular separation for samples in the sub-micrometer range via a field-flow-fractionation (FFF) channel.
Moreover, as the process is rapid and non-destructive, the sample remains unaltered by a stationary phase.
The Eclipse offers distinct advantages over conventional particle separation apparatus, like hydrodynamic chromotography (HDC) instruments. Only the Eclipse plus the Dawn can obtain particle sizes without the need for channel calibration with arbitrary standards, claims Wyatt.
The company has also incorporated various safety features into the design of the Eclipse, such as automatic shut-down and a pressure relief-valve actuation.
