Kit is said to provide >95% removal of albumin and IgG from 20ul of human serum, along with low non-specific binding of potential serum biomarkers
Vivascience has expanded its Vivapure sample preparation product line by introducing a new human albumin and IgG depletion kit.
The Vivapure Anti-HSA/IgG Kit utilises a combination of a unique Anti-HSA antibody fragment and Protein G resin for specifically depleting albumin and IgG from human samples.
The kit is said to provide >95% removal of albumin and IgG from 20ul of human serum, along with low non-specific binding of potential serum biomarkers.
Albumin and IgG depleted samples are recovered in a low salt buffer, facilitating downstream applications.
The method is described as quick, suitable for parallel processing, and offered in a ready-to-use kit.
The range of Vivapure sample preparation products includes Vivapure Anti-HSA Kit for albumin depletion, Vivapure Anti-HSA Resin for albumin depletion from serum volumes up to 400ul, and Vivapure Ion Exchange spin columns for sample fractionation.
The new Vivapure Kit further improves the resolution and detection of lower abundance sample proteins by electrophoresis, chromatography, and mass spectrometry by removing up to 75% of high abundant serum proteins.
The Anti-HSA ligand also shows reactivity against rat and mouse albumin.
As Protein G is also efficient at binding rat and mouse IgG, this kit can be utilised for processing samples from the three most frequently encountered sample sources in biomarker research.
The Vivapure Anti-HSA/IgG Kit contains Anti-HSA/IgG Affinity Resin, centrifugal columns which can individually be filled with the resin amount necessary, micro-centrifuge tubes, and binding buffer for preparing 12 serum samples.
The spin column format allows the processing of a single sample within 20 minutes and is just as convenient for the parallel processing of several samples simultaneously using standard laboratory equipment.
