Technical paper describes a novel flow cytometric type assay that provides rapid, accurate determination of Baculovirus titres
Guava Technologies has produced a new technical paper describing a novel flow cytometric type assay that provides rapid, accurate determination of Baculovirus titres.
Recombinant protein expression using the Baculovirus vector system is a powerful tool for the production of therapeutics, diagnostics and reagents.
To maximise efficiency of protein production, and thereby reduce costs, optimal manufacturing parameters are typically determined using the Baculovirus titre assay.
The technical paper discusses the benefits and shortfalls of traditional Baculovirus titre methods including real-time PCR, end point dilution and plaque assays.
Results obtainable by these methods are compared to the flow cytometric type assay carried out using a Guava PCA-96 system.
This is described as an affordable, ultra-compact system ideally suited for automatic, 96-well microplate and multiple microcentrifuge tube analysis of cell lines and compounds.
Intuitive in operation, the Guava PCA-96 provides precise, accurate and easy-to-read results with often less than one day of training.
The Guava PCA-96 system simplifies cell monitoring and screening, only a few microlitres of samples are required for each experiment, saving precious cells and reagents and reducing potentially hazardous biological waste.
Using a selection of Spodoptera frugiperda and Trichoplusia insect cell lines the flow cytometric type assay, performed on the Guava PCA-96, was found to give results of equal or greater accuracy than the traditional methods.
The independently researched technical paper concludes that the high-throughput Baculovirus titre, using the Guava PCA-96, facilitated accurate virus titre determination of multiple conditions in less than 24 hours, far less than the four days required for traditonal plaque assays.
Further, not only did the new assay enable determination of infectious particles versus viral transcript, the high-throughput format also allowed for direct comparison of various titre specific to target cells as well as media consumption.
