By utilising a variety of laboratory methodologies, these kits - which are now available throughout Europe - are able to detect distinct morphological changes in the apoptotic process
LGC Promochem announces that the new range of ATCC kits for apoptosis detection is now available throughout Europe.
Designed for the straightforward detection of this complex process, researchers are now able to detect all stages of the process quickly and reliably in cultured cells or tissue samples.
Over recent years, apoptosis has become an increasingly important area for life science researchers, comprising 2% of the scientific papers published.
Aimed at selectively removing excess or aged cells, it is a highly ordered biochemical process, which in turn, results in distinct morphological changes.
This stepwise mechanism lends itself to a number of detection methods - a fact conveniently exploited by the ATCC product range.
By utilising a variety of laboratory methodologies, the kits are able to detect distinct morphological changes in the apoptotic process.
The following kits are now available to order from LGC Promochem:.
Mitochondrial membrane potential assay kit - detects the breakdown of mitochondrial membrane potential seen in early apoptosis.
Caspase inhibitor kit - uses peptides to bind to activated caspase, an early and important indicator of apoptosis.
Caspase substrates kit - uses a cresyl violet label coupled to two caspase-specific peptides for powerful detection method that is cell-permeable and non-cytotoxic.
Parp kit - detects poly (ADP-ribose) polymerase, a nuclear protein active in DNA repair.
Annexin V kit - used to bind phosphatidylserine, a substance known to be redistributed to the outer surface of the cytoplasmic membrane during apoptosis.
Histone phosphorylation kit - uses a polyclonal antibody to identify a form of histone H2Ax, an indicator of double-stranded DNA breaks.
DNA laddering kit - late in the apoptotic process, cleaving of chromosomal DNA by endonucleases generates a series of DNA fragments that can be analysed by gel electrophoresis.
For added flexibility, researchers can choose products based on their preferred detection method: fluorescence microscopy, flow cytometry, multiwell plate fluorometry, gel electrophoresis, immunochemistry and radiometric analysis.
LGC Promochem also offers a full range of ATCC human and mouse clone sets containing all the genes identified to date that specifically participate in the apoptotic process.
These are provided in bacterial hosts for convenient scale-up and storage.
The ATCC apoptosis range is completed by quality inducible apoptotic human cell lines and cell line model systems.
Fully tested for quality, usability and performance, life science researchers can be confident of the reliability and reproducibility available within the ATCC apoptosis detection range.
As a non-profit biological resource centre that distributes research materials to scientists, ATCC began distributing cell lines in 1959, and, since then, has expanded to offer a wide range of media, sera, reagents and kits.
The apoptosis product line is an extension of the organisation's renowned expertise in cell growth.
