Superparamagnetic beads can fractionate mixed protein samples quickly and easily for downstream analysis such as mass spectrometry
Dynal Biotech has announced the release of the first two products in its new proteomic preparation range - weak cation exchange (WCX) Dynabeads and reversed phase chromatography (RPC) Dynabeads.
These novel beads are said to be an excellent alternative to chromatography columns, spin columns and pipette sample preparation technologies.
They can fractionate mixed protein samples quickly and easily for downstream analysis such as mass spectrometry.
These superparamagnetic beads can be used to concentrate, prepare and desalt complex protein mixtures.
The Dynabeads methodology is not only far quicker and easier to perform, but is also incredibly flexible and can be automated on any device, says the company.
Dynabeads WCX and Dynabeads RPC Protein are designed for the fractionation of intricate mixtures of protein (eg, serum, plasma, urine or cell lysates) based on different parameters: Dynabeads WCX carry surface charges which reversibly adsorb oppositely charged proteins and large peptides.
Dynabeads RPC Protein have a coating with optimised hydrophobic and hydrophilic components for peptide/protein binding.
The Dynabeads act as a controllable solid phase support, and respond to a magnetic field allowing bound material to be rapidly and precisely separated from salts and other contaminants.
Fractions are then released via controlled step-wise elutions.
For Dynabeads WCX this is achieved using increasing concentrations of salt or by changing the pH.
Dynabeads RPC Protein use hydrophobic interactions and therefore increasing the concentration of acetonitrile will release protein fractions.
Since the beads use different properties the two can be used to isolate different fractions from the same sample.
This means that they provide a powerful and adaptable preparation protocol for downstream mass spectrum analysis, HPLC and 1D/2D gel electrophoresis.
Dynal Biotech says all Dynabeads are highly engineered to provide uniform spheres with even magnetic properties and maximum surface area.
They remain dispersed in solution but very quickly move in response to a magnetic field, with no magnetic remanence.
The beads have been designed to accommodate many different surface chemistries and have been developed in a number of different sizes to suit different genomic, proteomic and in vitro diagnostic applications.
