Side scatter feature allows researchers to analyse whole blood for different types of white blood cells, without having to stain or fix the cells
Guava Technologies has introduced a new side scatter option for its EasyCyte system which, it says, makes single-cell analysis with accurate cell enumeration more versatile and easier than ever before.
The side scatter feature allows researchers to analyse whole blood for different types of white blood cells, without having to stain or fix the cells.
The granular structures in the cytoplasm of granulocytes, a type of white blood cell, cause more scattering of incident light than the more uniform cytoplasm of lymphocytes, another type of white blood cell.
This makes it easier to perform a three-part differential on whole blood - assessing the percentage and number of granulocytes, monocytes, and lymphocytes in a given sample.
"The addition of side scatter will expand our opportunities in the immunology and oncology markets," said Larry Bruder, Guava vice president, worldwide marketing and business development.
"Addition of side scatter functionality supports our goal to bring accessible and powerful, yet cost-effective cytometry to the life sciences market".
Side scatter is also used for a variety of others applications within the life sciences market, such as a QC measure for cell line sample integrity, as a way to eliminate debris from a sample, or to discern infected from non-infected cells.
The EasyCyte is a five-parameter system that Guava says is flexible, highly affordable, and ultra-compact.
Since it requires only a few microlitres of sample volume, it saves precious and expensive cells, reagents, and compounds.
The system is so easy to use, results are generated with less than a day's training.
EasyCyte runs each of Guava's turnkey assays, enabling easy, fast measurements of cell counting/viability, GFP expression and viability, apoptosis, cell cycle analysis, antigen detection, cell tracking, and more.