Disc centrifuge could be a critical tool for purification development of viral vectors for meeting therapeutic and research needs
Recombinant adenovirus is one of the primary vectors for human gene therapy.
However, the aggregation of unstable virus has been a recurring problem during production and purification of virus for human therapeutics.
To facilitate development of a robust manufacturing process for recombinant adenovirus vectors, a high resolution particle size distribution analytical tool is necessary.
Analytik says the CPS disc centrifuge is ideal for this application.
The CPS disc centrifuge can detect virus concentrations down to 0.01% (w/v) or 3x10^11 particles per ml.
The apparent hydrodynamic diameter of recombinant adenovirus was determined to be about 0.079um.
The disc centrifuge analysis was able to detect adenovirus aggregates (dimers, trimers, and tetramers) consistent with a rigid sphere approximation for adenovirus, as well as a large aggregate of 0.65um.
The technique could be highly effective in monitoring the kinetics of aggregation for adenovirus and other DNA and RNA viruses in the sub micron region.
Therefore the CPS disc centrifuge can be a critical tool for purification development of viral vectors for meeting therapeutic and research needs.
The CPS disc centrifuge can also be a useful tool in a very wide range of other particle size distribution analysis.
For example: virus particles/virus-like particles, cells (culture) and cell fragments, protein clusters, liposomes, particles in diagnostic tests, micro-encapsulated drugs, polymer latexes and emulsions.
