HPLC analysis of triterpene glycosides in dietary supplement formulations is the subject of an application note from Polymer Laboratories.
HPLC analysis of triterpene glycosides in dietary supplement formulations is the subject of an application note from Polymer Laboratories.
Currently, there is a strong initiative to establish industry-wide standards to ensure that dietary supplements, such as black cohosh, are manufactured consistently as to identity, purity, quality, strength and composition.
Black cohosh root can be taken as a dietary supplement to relieve the symptoms of the menopause.
Its primary active constituents are the triterpene glycosides, actein, 27 deoxyactein and cimifugoside, while biologically active substances, including alkaloids, flavonoids and tannins are also thought to contribute to the herb's potency.
This application note illustrates how evaporative light scattering detection is a reliable and accurate analytical technique for the analysis of triterpene glycosides.
These compounds possess weak or no UV chromophores, which limits their sensitivity and the ability to run gradient elution.
