This versatile diode array instrument has a comprehensive DNA/RNA application program built in, but can also be used as a standard spectrophotometer.
Hitachi High-Technologies has introduced the U-0080D Bio- spectrophotometer.
Sample volumes as small as 1ul can be quantitatively analysed to avoid wastage of valuable sample material.
The nucleic acid measurement package includes absorbance ratio (A260/280), purity and concentration measurements for RNA, ssDNA and dsDNA.
Oligonucleotide molar concentrations can also be calculated.
Protein concentration is calculated using the Warburg-Christian method.
A choice of background correction methods is provided.
Using diode array detection, measurements are extremely rapid.
An entire spectrum from the full 190-1100nm wavelength range of the instrument can be scanned in just 0.05 seconds.
The U-0800D can also be used for labelled DNA measurements.
By calculating the concentration of unlabelled primer (oligomar) and primer labelled with a fluorescent dye, the instrument can be used to verify that the dye is bound to the primer in advance of DNA sequencing applications.
The new instrument is a fully functional UV/vis spectrophotometer, with wavelength scanning, time scanning and full quantitative analysis capabilities.
Comprehensive spectral processing allows up to 20 spectra to be displayed simultaneously.
For quantitative analysis, up to 20 standard samples can be measured to create a working curve.
Samples can be measured up to five times, with calculation of standard deviation and coefficient of variance.
Spectral data can be saved for export directly into Microsoft Excel for further processing, if required.
