Guava Technologies has announced a new applications note that describes a novel multiplex approach to cellular analysis using its microplate-based Guava Easycyte system
The ability to obtain multi-parameter information on compound behaviour in the context of a living cell, quickly and easily, is invaluable to the drug discovery process.
This application note describes results for over 200 test cytoactive compounds in which the microcapillary-based Guava Easycyte system was used to measure Caspase 3/7 and 8 activity, mitochondrial membrane potential flux, phosphatidylserine translocation, membrane permeability and cell cycle disruption.
The reported results demonstrate how this combination of assays run on the Guava Easycyte system can be used to identify both cell cycle inhibitors and apoptotic inducers, as well as characterise their EC50 and quantify the percentage of cells affected.
Further the detection of sub-population effects for these compounds highlights the benefits of the Guava platform in analysing populations at the single cell level.
The novel multiplex assay approach on the Guava Easycyte system is demonstrated to offer a better understanding of the mechanism of action for a given compound and to provide more information concerning potential side effects such as potency, toxicity and specificity.
The Guava EasyCyte is a five-parameter microplate cytometer that is flexible, highly affordable, and ultra-compact.
Since it requires only a few microlitres of sample volume, it saves precious and expensive cells, reagents, and compounds.
The system is so easy to use, results are generated with less than a day's training.
Current screening assays available for the Guava Easycyte systems include cell counting and viability, cell marker expression, cytotoxicity, cell cycle, CD4/CD8 absolute counting and a suite of assays for apoptosis assessment and cell tracking.
Guava Technologies is a privately held biotechnology and medical device company and a leading provider of on-demand, easy-to-use single cell analysis systems.
Guava Personal Cell Analysis (PCA) systems, including the Guava PCA, Guava PCA-96 and Guava EasyCyte systems, are integrated, fully optimised, micro-capillary cytometry systems with embedded absolute cell counting capability.
Used worldwide by the life sciences, biotechnology, and pharmaceutical industries, as well as clinical testing institutions (outside the United States and Europe), Guava's products have broad applications in scientific research and throughout the drug discovery and lead optimisation process, as well as for cell counting and optimisation of commercial bio-pharmaceutical production.
Guava offers a variety of assays and dedicated software modules for the Guava PCA systems, enhancing the system's overall ease-of-use.
For a copy of the application note please contact Guava Technologies.
