This experiment introduces students to two important analytical concepts: fluorescence spectroscopy and kinetics as analytical method
While there are many 'standard' experiments performed using data acquisition with MeasureNet, the system's high precision and research grade probeware enable exciting flexibility and innovation in curriculum development.
In this first of a series of novel and adapted experiments for MeasureNet, the company's Bjoern Carle spells out a method to use the network's multifunction, dual-beam colorimeter to determine vitamin B1 (thiamin) content in commercially available tablets utilizing the interface's 'fluorescence kinetics' mode.
This experiment introduces students to two important analytical concepts: fluorescence spectroscopy and kinetics as analytical method.
The biochemical context of vitamin analysis makes this simple experiment useful for a wide range of classes.
In this study, the oxidation of non-fluorescent thiamine to its fluorescent oxidation product thiachrome (TC) is followed.
Initial rates of the increase in fluorescence are determined and quantitatively compared to a previously established calibration curve.
The analysis of a vitamin B1 tablet from Walgreens Pharmacy yielded a measured TM concentration of 46.7x10-6mol/L (+/-3%), compared to an expected 47.9x10-6mol/L of TM.
The TM concentration of a vitamin B1 tablet from Kunkel Pharmacy was analysed to 43.4x10-6mol/L (+/-0.7%), compared to an expected concentration of 45.8x10-6mol/L.
The results shown indicate that the MeasureNet Colorimeter is well suited for the analysis of vitamin B1 using this initial rate method.
Contact MeasureNet for the full experimental procedure.
